Ua hoʻohana nā mea noiʻi i ka pipeline stable isotope probing (HT-SIP) hou a me ka metagenomics e kiʻi mua i ka microbiome ikaika e hoʻopuni ana i kahi symbiont mea kanu pono, arbuscular mycorrhizal fungi (AMF). Loaʻa ʻia: Lawrence Livermore National Laboratory
ʻO ka hoʻopili ʻana i ka ʻike o nā microbes hihiu me ko lākou ʻano physiological a me nā hana kaiapuni kahi pahuhopu nui no nā microbiologists kaiapuni. ʻO nā ʻenehana e hoʻoikaika nei no kēia pahuhopu, ʻo Stable Isotope Probing—SIP—ua manaʻo ʻia ʻo ia ka mea maikaʻi loa no ke aʻo ʻana i nā microorganism ikaika i nā ʻano kūlohelohe.
Ua hoʻomohala nā ʻepekema ʻo Lawrence Livermore National Laboratory (LLNL) i kahi ʻenehana hou-high-throughput SIP-e hoʻokaʻawale i nā ʻanuʻu he nui i ke kaʻina o ka hoʻāʻo ʻana i ka isotope paʻa, e ʻae ana i nā noiʻi o ka hana microbial o nā microorganism ma lalo o nā kūlana maoli, me ka ʻole o ka pono o ka culturing lab.
Ma SIP, ʻike ʻia nā microbes ikaika ma o ka hoʻohui ʻana i nā isotopes paʻa i loko o kā lākou biomass. ʻO ia kekahi o nā ala ikaika loa i ka microbial ecology no ka mea hiki iā ia ke ʻike i nā microbes ikaika a me ko lākou mau ʻano physiological (hoʻohana substrate, biochemistry cellular, metabolism, ulu, make) i nā kaiāulu paʻakikī ma lalo o nā kūlana maoli.
ʻO ka maʻamau, pono ke ʻano SIP i ka hana lima lima a ʻae wale i kahi helu liʻiliʻi o nā laʻana. Akā ʻo ka ʻenehana LLNL hou e koi i hoʻokahi hapaono o ka nui o ka hana lima lima i hoʻohālikelike ʻia i ka SIP manual a hiki ke hana ʻia nā laʻana 16 i ka manawa like.
"ʻO kā mākou ala semi-aunoa e hoʻemi i ka manawa o ka mea hana a hoʻomaikaʻi i ka hana hou ʻana ma o ka huli ʻana i nā hana koʻikoʻi o ka SIP," wahi a ka mea ʻepekema ʻo LLNL ʻo Erin Nuccio, a me ka mea kākau alakaʻi o kahi pepa i hōʻike ʻia ma ka puke pai Microbiome. "Ua hoʻohana mākou i kēia ala no ka hoʻoponopono ʻana ma luna o hoʻokahi kaukani hoʻohālike, me kekahi o nā microhabitats lepo ʻike ʻole."
ʻO kekahi o ia microhabitat ʻo ia ka lepo e hoʻopuni koke ana i nā ʻiʻo o mycorrhizae—he ʻano ʻano haʻuki e hana i nā pilina symbiotic me 72% o nā mea kanu ʻāina āpau. Ma ke kūʻai ʻana i ke kalapona mea kanu, hāʻawi ka haʻo (arbuscular mycorrhizal fungi) i kona mau pūʻali me nā kumuwaiwai koʻikoʻi e like me ka nitrogen, phosphorus a me ka wai.
Ma kēia haʻawina hōʻoia o ka manaʻo, ua hōʻike nā mea kākau i ka "pūnaewele meaʻai" o nā pilina i hoʻoulu ʻia e nā fungi mycorrhizal i ka lepo.
“Manaʻo mākou he ala nui kēia no ka laha ʻana o ke kalapona kanu i ka lepo. Paʻa ka lepo i ka wai puna nui loa o ke kalapona kūlohelohe i ka honua, "wahi a ka mea kākau like ʻo Jennifer Pett-Ridge, ʻo ia ke alakaʻi o ka papahana LLNL a me ke poʻo o ke Keʻena ʻOihana o Energy "Microbes Persist" Soil Microbiome Scientific Focus Area. . "Ua hoʻonohonoho mākou i kahi helu liʻiliʻi o DNA, hoʻoholo i nā mea ola kino a laila kūkulu hou i kā lākou genomes a me nā pilina pili."
ʻO nā mea kākau LLNL ʻē aʻe ʻo Steven Blazewicz, Marissa Lafler, Ashley Campbell, Jeffrey Kimbrel, Jessica Wollard, Rachel Hestrin a me nā mea noiʻi mai Lawrence Berkeley National Laboratory, ka DOE Joint Genome Institute a me ke Kulanui o Kaleponi, Berkeley.